45346 甘露醇卵黄多粘菌素琼脂 BR 250g 135
称取本品46g，加入蒸馏水或去离子水1 L，搅拌加热煮沸至完全溶解，分装三角瓶，121℃灭菌15分钟。冷至50℃, 每100ml培养基中加入50%卵黄液5ml和多粘菌素B(082202)10000IU，混匀后倾注灭菌培养皿。Suspend 46 g in 1 L of distilled or deionized water.Heat with frequent agitation and boil to completely dissolve the powder.Distribute into flasks.Autoclave at 121℃ for 15 minutes and then cool to 50℃.Add 5 ml of 50% sterile egg-yolk emulsion and 10000IU of polymyxin B to 100 ml of the medium.Mix thoroughly and pour into sterile Petri dishes.
蛋白胨Peptone.............................10g 牛肉膏粉Beef extract......................1g D-甘露醇D-mannitol........................10g 氯化钠Sodium chlorode.....................10g 琼脂Agar..................................15g 酚红Phenol red............................0.025g 最终pH Final pH...........................7.4±0.2 有效期：三年 Period of validity:Three years.

用于蜡样芽孢杆菌的菌数测定及分离培养。For isolating and enumerating Bacillus cereus.